Effect of a Triple Anti-Enteroviral Combination via Consecutive Alternating Administration in Experimental Coxsackievirus B3 Infections in Mice
Abstract from Fourth National Congress of Virology with International Participation /Days of Virology in Bulgaria Sofia, May 18th - 20th, 2016
Angel S. Galabov The Stephan Angeloff
Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
In our previous studies, the efficacy of consecutive alternating
administration (CAA) of the triple combinations pleconaril /guanidine/oxoglaucine
and pleconaril/MDL-860/ oxoglaucine against Coxsackievirus B1 (CVB1) infection
in newborn mice was proven. It was shown that these drug combinations prevented
the development of drug resistance in virus progeny.
In the present study, we test the effect of the triple
combinations described above on experimental infections in newborn mice
infected subcutaneously with 20 MLD50 of two strains of Coxsackievirus B3 –
neurotropic (Nancy) and cardiotropic (Woodruff). The results of these analyses
indicate markedly improved efficacy of PGO and PMO combinations administered
according to the CAA treatment schedule in CVB3 infected mice – decreased mortality
rate and lengthening of the mean survival time (MST). In comparison with
placebo groups the monotherapeutic courses with pleconaril and MDL-860
demonstrated some independent antiviral effect. It was found that oxoglaucine and
guanidine.HCl monotherapies were without a marked antiviral effect.
These results add new support for the use of CAA treatment
courses in achieving clinically effective chemotherapy of EV infections.