Inhibition Effect on Coxsackievirus B3 and Poliovirus Type 1 Replication by Combining of MDL-860 with other Enterovirus Inhibitors

Abstract from Fourth National Congress of Virology with International Participation /Days of Virology in Bulgaria Sofia, May 18th - 20th, 2016

Adelina Stoyanova, Lora Nikolova, Angel S. Galabov

The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

The use of single anti-enteroviral drugs (monotherapy) to inhibit enterovirus replication is always problematic because resistance usually arises rapidly. Combination of drugs with different mechanisms of action was required for maximal suppression of replication, resulting in reduced rate of resistance. In this study, we observed the effects of MDL-860, HBB and guanidine.HCl in pair combinations on poliovirus type 1 (LSc-2ab) and Coxsackievirus B3 (Nancy) replication in HEp-2 cells were tested. The fifty per cent inhibitory concentration (IC50) was determinate for each compound and virus.

Combining MDL-860 with HBB and, MDL-860 with guanidine.HCl resulted in additive interaction on PV1 replication. The effect of MDL-860 with guanidine.HCl on CVB3 replication was also additive, while MDL-860 with HBB demonstrated moderate synergistic effect.