Phylogenetic Analysis of Ukrainian Isolates of RNA Viruses of Plants
Abstract from Fourth National Congress of Virology with International Participation /Days of Virology in Bulgaria Sofia, May 18th - 20th, 2016
Iryna Budzanivska, Tatyana Shevchenko, Olexiy Shevchenko, Valeriy Polishchuk
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
The availability of analytical approaches allowing fast deciphering of nucleotide sequences enables phylogenetic analysis of viruses and establishing homology among their strains/isolates for unveiling the history of virus evolution.
This work was focused on phylogenetic analysis of most important viruses infecting fruit, vegetable and cereal crops in Ukraine. ‘Field’ isolates of different RNA viruses of plants were collected and analyzed from various agriecosystems. These included Cucumber green mottle mosaic virus (CGMMV), Plum pox virus (PPV), Potato virus Y (PVY), Potato virus X (PVX), Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Cucumber mosaic virus (CMV), Watermelon mosaic virus 2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PepMMV), and Wheat dwarf virus (WDV), etc.
Using MEGA5 software, we constructed phylogenetic trees basing on sequenced viral cDNAs corresponding to parts of coat protein genes of studied pathogens. Differing evolutionary trajectories were shown for some of Ukrainian virus isolates.
Study of molecular properties of viruses, and especially those of viral genomes, has fundamental importance (tracing phylogenetic relationships of a given virus with other members of the kingdom, its evolutionary history and trends) but is also of practical significance as such data may further be used both for developing highly specific PCR-based diagnostic techniques, and for forecasting possible alterations and novel properties of circulating viruses for prevention of disease spread and minimizing the aggressiveness of viruses.